Bioactive Scaffolds Based on Amine-Functionalized Gellan Gum for the Osteogenic Differentiation of Gingival Mesenchymal Stem Cells

With the aim to produce a cellularized construct for guided bone regeneration of dento-alveolar defects, here we porous scaffold using an amine derivative gellan gum host gingival mesenchymal stem cells (GMSCs) and allow their osteochondral differentiation. Three derivatives were produced by same synthetic procedure, viscoelastic properties aqueous dispersions investigated compared those native polysaccharide choose with suitable production. Freeze-drying was used obtain sponge that can be rehydrated cells’ suspension implantable cell containing hydrogel. We cytocompatibility gum-((2-aminoethyl)-carbamate) (named GG-EDA) possibility use it as starting biomaterial able support osteogenic differentiation GMSCs. Our data suggest GG-EDA did not negatively affect proliferation nor osteo-inductive capability